Jeng Fan Lo

WORK ADDRESS

Institute of Oral Biology, School of Dentistry, National Yang Ming Chiao Tung University

No.155, Sec. 2, Li-Nong St., Pei-Tou, Taipei (112), Taiwan, R.O.C.

email: jflo@nycu.edu.tw

Tel: 886-2-2826-7222 Fax: 886-2-2826-4053

Summary of CAReer goal

I am a research scientist with experienced training in the areas of toxicology, protein biochemistry, mouse genetics, oncology and stem cell biology.  I plan to use my diverse training to research the mechanisms of biomedical disease. My goal is to become an independent and successful principal investigator in biomedical research. Ultimately, I want to develop successful therapeutic regimens for disease treatment including cancer, ageing and obesity etc.

research interest

Cancer Biology, Stem Cell Biology, Mitochondrial Dysfunction, Mouse Genetics, Molecular Immunology, Animal Disease Models and Therapeutic Regimen Development

 

Education

 

1994-1999               Ph.D., Department of Microbiology and Immunology, University of Illinois at Chicago. Thesis: Characterization of a silver binding protein (SilE) from the genes of Salmonella (Thesis advisor: Dr. Simon Silver).

 

1988-1990              M.Sc., Fu-Jen Catholic University, Taiwan. Thesis: Glutathione S-transferase p in an arsenic resistant cell line: Characterization and its role on arsenic resistance. (Thesis advisor: Dr. Te-Chang Lee).

 

1984-1988              B.Sc., Fu-Jen Catholic University, Taiwan

 

 

Employment 

 

2019 Aug -  now         Distinguished Professor at National Yang Ming Chiao Tung University

 

2018 Aug -  now         Faculty of Institute of Biochemistry and Molecular Biology, School of Life Science, National Yang Ming Chiao Tung University

 

2018 Aug -  now         Faculty of Department and Institute of Pharmacology, School of Medicine, National Yang Ming Chiao Tung University

 

2014 Aug -  2020 Jul  Director of Institute of Oral Biology, School of Dentistry, National Yang-Ming University

 

2011 Aug -  now            Professor at Institute of Oral Biology, School of Dentistry, National Yang-Ming University.

 

2011 Aug -  now           Adjunct Professor at Department of Dentistry, School of Dentistry, National Yang-Ming University.

 

2011 Aug -  now           Adjunct Professor at Graduate Institute of Basic Medical Science, College of Medicine, China Medical University.

 

2008 Aug -  now           Adjunct Research Fellow at Department of Dentistry, Taipei Veterans General Hospital.

 

2005 Aug -  2011 Jul    Associate Professor at Institute of Oral Biology, School of Dentistry, National Yang-Ming University.

                                   

2008 Aug -  2011 Jul    Adjunct Associate Professor at Department of Dentistry, School of Dentistry, National Yang-Ming University.

 

2000 Jan -  2005 Mar    Research Associate (Postdoctoral Fallow) at The Scripps Research Institute, La Jolla, CA. USA. Research project: Understanding the biological function of a putative mammalian tumor suppressor through generation of a conditional knockout mice system to set up a novel cancer screen system in the future.

QUALIFICATIONS:

 

·      Experienced in generating conditional knockout mice.

·      Experienced in studying the function of a putative tumor suppressor in T cell development from T-cell-specific conditional knockout mice.

·      Experienced in studying the function of a putative tumor suppressor in hepatocyte development and liver development from liver-specific conditional knockout mice.

·      Familiar with mouse in vivo transgenic or chemical carcinogenesis models of cancer research: skin cancer (DMBA and TPA two-stage carcinogenesis), liver cancer (DEN or aflatoxin B1 carcinogenesis), lung cancer (transgenic k-ras carcinogenesis), breast cancer (transgenic Wap-Tg, mmtv-Her or MNU carcinogenesis) and prostate cancer (transgenic TRAMP carcinogenesis).

·      Experienced in using small interfering RNA (siRNA) to down regulate gene expression in cell lines and chicken embryo.

·      Experienced with using DNA Microarray and Quantitative RT-PCR to evaluate gene expression in mammalian cancer research.

·      Proficient in molecular cloning and cell culture techniques.

·      Proficient at using the yeast two-hybrid system to screen new targets.

·      Experienced at using over-expression and purification of proteins from prokaryotic or eukaryotic system.

·      Experienced with molecular biological (site direct mutagenesis) and biochemical methods (CD, AAS, NMR and fluorescence spectroscopy, etc.) to characterize purified proteins.

·      Experienced at conducting protein structure-function studies using either crystallization or NMR.

 

Major research experience:

 

1. Isolation and characterization of cancer stem-like cells in oral cancer: Enrichment of cancer stem-like cells from oral cancer cells or primary oral cancer tissues by serum-free medium plus bFGF and EGF growth factors or side population cells (Hoechst33258 dye exclusion). Establishment of radioresistant oral cancer cells by progressive selection.

 

1. Generation of different tissue specific Tid1 knockout mice by breeding Tid1-floxed mice with individual Cre transgenic mice (Lck-Cre, CD4-Cre, Alb-Cre, K14-Cre, WAP-Cre, mmtv-Cre, Syn-Cre or MX-Cre) to understand the function of DNAJA3 (Tid1) on different tissues: total RNA preparation, DNA microarray, RT-PCR, MTS or CFSE cell proliferation, flow cytometery, MTT assay, coimmunoprecipitation, Western blot, Southern blot, immunochemical staining (confocal microscopy), histology assay, mouse anatomy, blastocyst outgrowth, T cell isolation, hepatocyte isolation and keratinocyte isolation.

 

1. Conditional transgenic mice establishment to study the function of DNAJA3 (Tid1) as a tumor suppressor: Genomic database search, bioinformative search, design of gene targeting vector, construction of gene targeting plasmids, screening of gene of interest from BAC genomic library, primary mouse embryonic fibroblast (MEF) and embryonic stem cell (ES) culture, DNA electroporation on ES cells, stable undifferentiated ES cell line establishment, genomic DNA preparation of ES cell, screening of homologous recombination on mouse ES cell by Southern hybridization and genomic PCR, targeted mice germ lines selection, and adenovirus or retrovirus gene delivery.

 

1.   Down regulation of genes expression upon inhibition of BMK1 kinase by RNA interference in cancer cell lines to elucidate the function of BMK1 on tumorigenesis: Utility of siRNA technique to down regulate the posttranscriptional mRNA of BMK1 kinase, MTT assay, total RNA preparation, DNA microarray, flow cytometery, coimmunoprecipitation and Western blot.

 

1. Yeast two-hybrid to screen the erbB2 interacting proteins involving in the signal transductions of breast cancer cells: Cloning of genes of interest for yeast two-hybrid screening, yeast two-hybrid screening on high throughput 96- well format, DNA transfection of mammalian cell line, stable cell line establishment, rabbit polyclonal antibody generation, total mRNA extraction, MTT assay, b-galactosidase activity assay, adenovirus expression construct preparation and establishment, Western blot, RT-PCR, construction of cDNA library, deletion or site-directed mutagenesis, peptide mapping, phosphopeptide mapping, coimmunoprecipitation and cis or trans reporting assay (luciferase activity assay).

 

1. Cloning, expression, purification and characterization of a silver binding protein (SilE) from the genes of Salmonella: Cloning of SilE over expression strain by PCR, purification of SilE by ammonium sulfate precipitation, cation exchange chromatography and gel filtration or HPLC, peptide sequencing, circular dichroism (CD) to study the secondary structure change after the binding of Ag+ to SilE, inductive coupling plasma (ICP), atomic absorption spectroscopy and mass spectroscopy to study the stoichiometry of Ag+ binding to SilE, NMR (proton or HSQC) to study the change of imidazole groups of histidine residues of SilE that are responsible for Ag+ binding and replacement of tryptophan on phenylalanine of SilE by site-directed mutagenesis to study the binding constant of SilE to Ag+ through fluorescence spectroscopy.

 

1. Cloning, expression and purification of a DNA binding protein (CadC, renamed CadR) on the cadmium resistance mechanism in bacteria (Bacillus, Staphyl and methicilline resistant Staphyl): Cloning of GST-cadC fusion strain by PCR, gel retardation, probe labeling, mass growth of bacteria by fermentation, GST-CadC purification by affinity chromatography, anion exchange chromatography and gel filtration.

 

1. Crystallization of mutant protein of CheY, a regulatory protein of the two-component system in E. coli: Cloning of expression plasmid, purification of over expressed protein by gel filtration (FPLC), affinity chromatography, ion exchange chromatography, SDS-PAGE, crystallization by hanging-drop and data collection on X-ray diffraction. 

 

1. Selection of transformed cell line by Bovine Papilloma Virus: Cell line transfection, cell line establishment from a single colony on soft agar.

 

1. Purification and characterization of Glutathione S-transferase p from an arsenic-resistant Chinese hamster ovary cell line: Cell culture, GST purification through affinity chromatography, 1D and 2D electrophoresis, characterization on enzymatic kinetics of GST-p by photospectroscopy, polyclonal antibody preparation against GST-p, Western blot, cytotoxicity assay by colony formation.

 

 

Teaching experience

 

2018 Feb - now                    Faculty of Department and Institute of Pharmacology, School of Medicine, National Yang-Ming University. Course: Experimental Pharmacology, Special Topic on Cancer Stem Cell, Molecular Immunology and Special Topic on Mouse Transgenic.

 

2011 Aug - now                   Professor at Institute of Oral Biology, School of Dentistry, National Yang-Ming University. Course: Advanced Biotechniques, Biochemistry, Cell Biology, Biomedical Research and Scientific Writing, Special Topic on Cancer Stem Cell, Molecular Immunology and Special Topic on Mouse Transgenic.

  

2005 Aug - 2011 Jul     Associate Professor at Institute of Oral Biology, School of Dentistry, National Yang-Ming University. Course: Advanced Biotechniques, Biochemistry, Cell Biology, Biomedical Research and Scientific Writing, Special Topic on Cancer Stem Cell, Molecular Immunology and Special Topic on Mouse Tragenics.

 

1996                                                Teaching assistant of Department of Microbiology and Immunology, School of Medicine at University of Illinois at Chicago. Course: Laboratory of Clinical Microbiology for 2nd year medical students.

 

1989                                                 Teaching assistant of Department of Biology at Fu-Jen Catholic University, Taiwan. Course: Laboratory of Comparative Animal Anatomy.

 

 

ReVIEWER of finding agency

 

2006 to Present     Primary grant reviewer of Dentistry, Basic Medical Science, Biological Science and Biochemistry and Pharmacology, National Science and Technology Council. 

 

2011 to Present     Panel of Dentistry, Basic Medical Science, Clinical Medical Science and Hematology, Oncology, Infection and Immunology, National Science and Technology Council.

 

2018                                Swiss Cancer League